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Twenty- four colonies are picked and cultured separately in 3 mL of Register 2 PCR amplification cycles and annealing temperatures/conditions Forward primer Verso primer *Annealing temp. seeking the leading 10 cycles (C) **Annealing temp. in behalf of 21 cycles (C) Burdensome bond 5 L-VH1 3 CCH1 6459 59 5 L-VH3 3 CCH1 6459 59 5 L-VH4/6 3 CCH1 6459 59 5 L-VH5 3 CCH1 6459 59 Kappa gyve 5 L-V1/2 3 C494 7267 67 5 L-V3 3 C494 7065 65 5 L-V4 3 C494 7065 65 Lambda train 5 L-VL1 3 C 7166 66 5 L-VL2 3 C 7166 66 5 L-VL3 3 C 6762 62 5 L-VL4/5 3 C 7166 66 5 L-VL6 3 C 6358 58 5 L-VL7 3 C 6762 62 5 L-VL8 3 C 6358 58 Beta-actin Actin F1379 Actin R1663 *Manage board 2 towards annealing temperature of primer in holy matrimony worn **The hang of inventory 2 for annealing temperature of primer two of a kind in use accustomed to Soung-chul Cha et al. 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